- Home
- China Products
- China Supplier Directory
- Focus Products : troponin i elisa , elisa microplate reader
| Categories | Human ELISA Kit |
|---|---|
| Place of Origin: | China |
| Brand Name: | DLdevelop |
| Certification: | CE, ISO9001:2015 |
| MOQ: | 48T |
| Price: | Negotiation |
| Packaging Details: | Carton Box |
| Delivery Time: | 1-2 Working Days |
| Payment Terms: | TT |
| Supply Ability: | Abundant Supply |
| Traditional ELISA Kit | Ready-to-Use ELISA KIT | |
| Product name: | Human Podocan (PODN) ELISA Kit | |
| Method: | Sandwich | |
| Synonyms: | PCAN; SLRR5A | |
| Detection range: | 78.125-5000pg/mL | |
| Reactivity: | PODN | |
| Size: | 96T/48T | |
| Quality guarantee period: | for 12 months | |
| Catalog number: | DL-PODN-Hu (traditional) | DLR-PODN-Hu (ready-to-use) |
| Assay length | 1-4.5Hours | 1-3.5Hours |
| Advantages: |
|
|
| Instruction Manual | ||
| Item | Standard | Test | |
| Description | The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PODN in human tissue homogenates or other biological fluids. | Conform | |
| Identification | Colorimetric | Positive | |
| Composition | Traditional ELISA Kit | Ready-to-Use ELISA KIT | Conform |
| Pre-coated, ready to use 96-well strip plate 1 | Pre-coated, ready to use 96-well strip plate 1 | ||
| Plate sealer for 96 wells 2 | Plate sealer for 96 wells 2 | ||
| Standard 2 | Standard 2 | ||
| Diluents buffer 1×45mL | Standard Diluent 1×20mL | ||
| Detection Reagent A 1×120μL | Detection Solution A 1×12mL | ||
| Detection Reagent B 1×120μL | Detection Solution B 1×12mL | ||
| TMB Substrate 1×9mL | TMB Substrate 1×9mL | ||
| Stop Solution 1×6mL | Stop Solution 1×6mL | ||
| Wash Buffer (30concentrate) 1×20mL | Wash Buffer (30concentrate) 1×20mL | ||
| Instruction manual 1 | Instruction manual 1 | ||
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PODN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PODN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
After TMB substrate solution is added, only those wells that contain PODN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm10nm.
The concentration of PODN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Matrices listed below were spiked with certain level of recombinant PODN and the recovery rates were calculated by comparing the measured value to the expected amount of PODN in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 84-99 | 91 |
| EDTA plasma(n=5) | 82-103 | 92 |
| heparin plasma(n=5) | 84-102 | 93 |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PODN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-96% | 83-98% | 81-99% | 93-101% |
| EDTA plasma(n=5) | 88-101% | 86-95% | 90-102% | 80-93% |
| heparin plasma(n=5) | 80-91% | 82-90% | 95-104% | 79-95% |
|
Human SH3 Domain-Binding Glutamic Acid-Rich-Like Protein 3（SH3L3） ELISA Kit
Human Acid Phosphatase, Prostatic (ACPP) ELISA Kit
Human Homing Associated Cell Adhesion Molecule (HCAM) ELISA Kit
Human Chordin (CHRD) ELISA Kit
Human Scleraxis Homolog (SCX) ELISA Kit
Human Mevalonate Decarboxylase (MVD) ELISA Kit
Diphosphoinositol Elisa Test Equipment Polyphosphate Phosphohydrolase 3 Beta
Human 5-Hydroxytryptamine Receptor 2B (HTR2B) ELISA Kit
Human 5-hydroxytryptamine Receptor 7 (HTR7) ELISA Kit
Human Deoxyribonuclease I Like Protein 3 (DNASE1L3) ELISA Kit
